Aging Negatively Affects Estrogens-Mediated Effects on Nitric Oxide Bioavailability by Shifting ERα/ERβ Balance in Female Mice (original) (raw)

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Figure 1

NO production in young (SAMR) and aged (SAMP) female mice.

(A) representative images of DAF2-DA fluorescence in mice aorta at basal level and after challenging with carbachol (Cb) 10 µM; (B) dose response, shown as relative green fluorescence intensity (fold increase) following carbachol stimulation; (C) nitrite/nitrate (NO2/NO3) concentration (µM) in plasma from SAMR and SAMP mice. OVX, ovariectomized; OVX+E2, ovariectomized treated with estrogen. Data are plotted as the mean ± S.E.M derived from 6–8 independent experiments. * p<0.05.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0025335.g001