Distinct Temporal and Anatomical Distributions of Amyloid-β and Tau Abnormalities following Controlled Cortical Impact in Transgenic Mice (original) (raw)
Figure 2
Intra-axonal Aβ accumulation monotonically increases from 1 to 24 hours post CCI in 3xTg-AD mice.
A. Aβ staining with biotinylated HJ3.4 antibody (against Aβ1–13) in the ipsilateral fimbria/fornix of a sham 3xTg-AD mouse. Sections were counterstained with Cresyl violet. Scale bar: 50 µm. B–F. Aβ staining in the ipsilateral fimbria/fornix of an injured 3xTg-AD mouse at 1 h (B), 6 h (C), 9 h (D), 12 h (E) and 24 h (F) after CCI. G. Stereological quantification of total numbers of Aβ-positive axonal varicosities as a function of time after injury in 3xTg-AD mice. N = 4–8 mice per group per time point. Bars represent mean ± SEM. One-way ANOVA with Newman-Keuls post tests, # p<0.05, ## p<0.01: significant increase from injured mice from previous time point. ** p<0.01, *** p<0.0001: significant increase from sham mice at same time point.