Notch Lineages and Activity in Intestinal Stem Cells Determined by a New Set of Knock-In Mice (original) (raw)
Figure 1
Notch1 and Notch2 expression in the small intestine.
X-gal staining (blue) of thin sections of proximal small intestine of N1-CreERT2SAT/+;R26R/+ (A) and N2-CreERT2SAT/+;R26R/+ (B) 24 hours after tamoxifen administration shows that Notch1 and Notch2 are expressed only in the intestinal crypts. C–D) In situ hybridization for Notch1 (C) and Notch2 (D) demonstrates specific expression in intestinal crypts. E–F) Whole mounts duodenum of N1-CreERT2SAT/+;R26R/+ (E) and N2-CreERT2SAT/+;R26R/+ (F) 7 months after tamoxifen administration demonstrates long-term labeling of X-gal marked Notch lineages. Counterstain: hematoxylin/eosin in A. Scale bar: 50 µm in A–D and 1 mm in E–F.