Isolation and Characterization of Renal Erythropoietin-Producing Cells from Genetically Produced Anemia Mice (original) (raw)

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Figure 2

GFP expression in the anemic kidney of EpoGFP/Δ3′E mouse.

Representative photos of P5 neonates: kidney slices A from a nonanemic EpoΔ3′E/wt littermate as a negative control; BD from EpoGFP/Δ3′E newborns. Light and confocal microscopy revealing the GFP signal concentrated in the deep-cortex∼outer-medulla region (B), where the Epo-producing peritubular interstitial cells are localized (C,D). Anti-GFP signals: green (Alexa 488), and dark-brown (diaminobenzidine). Scale bars: 25 µm. The sketch at the bottom illustrates peritubular localization of the GFP-expressing cells in the kidney.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0025839.g002