Isolation and Characterization of Renal Erythropoietin-Producing Cells from Genetically Produced Anemia Mice (original) (raw)
Figure 3
Cellular marker characterization of the GFP+ kidney cells of EpoGFP/Δ3′E mouse.
Dual immunostaining combined with confocal microscopy indicating GFP-expressing cells are not endothelial (CD31, A), dendritic (MHCII, B), and tubular cells (E-cadherin, Movie S1). GFP-positive interstitial cells express fibroblast-like markers (PDGFRβ, C), but not myofibroblast marker (α-SMA, D). Kidney slices from P5 EpoGFP/Δ3′E mice were stained with GFP (green) and the indicated antibodies directly labeled with phycoerythrin (PE, red) or indirectly labeled with Alexa 594 (red) respectively. Merged images of the same kidney section are shown. DAPI: blue/nucleus; Scale bars: 25 µm.