Pumilio-2 Function in the Mouse Nervous System (original) (raw)
Figure 3
Nesting behavior and stress.
(A–B) Nesting behavior. Nestlets were provided at night and nests were scored the next morning. (A) Examples for nest scoring: top left picture show score 5 (perfect) nest with walls higher than the mouse body for more than 50% of its circumference, bottom left picture shows score 2: Nestlet is only partially torn (50–90%), right hand picture shows score 3: less than 90% of the Nestlet is within a quarter of the cage floor area. (B) Pum2XE772 mice have impaired nest building abilities. The baseline effect is further (significantly) enhanced after surgery. (C) Corticosterone analysis in baseline (from feces) and after stress (serum). For baseline measurements, total corticosterone was extracted from feces collected from an 8-hour period. To analyze corticosterone levels under stressed, mice were restrained for 20 minutes and immediately sacrificed to collect blood. There was no significant difference between Pum2XE772 mice and wildtype littermates under either condition (***p<0.001).