Absence of Colony Stimulation Factor-1 Receptor Results in Loss of Microglia, Disrupted Brain Development and Olfactory Deficits (original) (raw)
Figure 4
Effects of the loss of the CSF-1R on cell populations in the cortex.
A. 5 µm sections of cortex (a–d, g,h) and hippocampus (e, f) immunostained with anti NeuN (a, b), anti-GFAP (c–f) or anti-Nogo A antibodies (g,h). c–h counterstained with Haematoxylin. Wild Type (a,c,e,g) and Csf1r−/− mice (b,d,f,h) Bar = 20 µm. B. Total cell counts (N = 3/genotype) determined as described in the materials and methods in the regions indicated in Figure 2B 5, 6 of all Nissl stained cells (a), NeuN positive cells (b), GFAP positive cells (c) and Nogo A positive cells (d). GFAP positive cells are increased in Csf1r−/− mice compared to WT (p = 0.022) while Nogo A positive ones are decreased (p = 0.0314).