Anti-Transforming Growth Factor ß Antibody Treatment Rescues Bone Loss and Prevents Breast Cancer Metastasis to Bone (original) (raw)
Figure 2
Anti-TGFβ antibody reduces osteolytic lesions in MDA-MB-231 breast cancer bone metastasis cardiac injection model.
Mice were inoculated with MDA-MB-231 human breast cancer cells in the left cardiac ventricle and were treated with either isotype control (13C4, 10 mg/kg) or anti-TGFβ antibody (1D11, 10 mg/kg) for 4 weeks, starting from 1 day post tumor cell injection. At the end of the experiment, whole body X-ray images of mice from both control and anti-TGFβ antibody treated group were taken and osteolytic lesion area and osteolytic lesion counts were analyzed using image analysis software (Metamorph, Molecular Device). Panel a: Representative X-ray images of osteolytic bone lesions in the hind leg of mice treated for 4 weeks either with control antibody (13C4, left panel) or anti-TGFβ antibody (1D11, right panel). White arrows indicate presence of osteolytic lesions. Panel b: A boxplot representing the average lesion counts in mice inoculated with MDA-MB-231 cells in the left cardiac ventricle, treated with either control antibody (13C4, 10 mg/kg) or anti-TGFβ antibody (1D11, 10 mg/kg) for 4 weeks, starting 1 day after tumor cell injection shows decrease in lesion numbers after anti-TGFβ treatment (6.9±1.7 for control and 1.9±0.7 for 1D11; Wilcoxon rank-sum p-value = <.001, N = 9). Panel c: A boxplot representing the lesion area from the same experiment shows decrease in the lesion area after anti-TGFβ treatment (20520±6000 for control and 1497±888 for 1D11; Wilcoxon rank-sum p-value = <.001, N = at least 9). Lesion areas were measured using arbitrary pixel unit.