Mll5 Is Required for Normal Spermatogenesis (original) (raw)
Figure 5
Impaired spermatozoa of homozygous Mll5tm1Apa mice cannot be rescued by in vitro fertilization.
(A) In vitro fertilization experiments were performed as described. Arrows indicate male pronuclei (in 10 hr panel) and fertilized embryos (in 48 hour panel) as seen under phase contrast microscopy. The data and percentages are given in Table 3. (B) Embryos flushed from the oviducts of naturally mated females with wild type and -/- males respectively at day 0.75 and were stained with PI (red; DNA) and anti-methyl-Cytosine (green; methylated DNA) and imaged by fluorescence microscopy. Sperm (white arrowheads) can be seen binding to the embryos in both cases, whereas fertilization indicated by the methylation pattern of the two pronuclei is only evident in wild type mated mice.