Tumor-Derived G-CSF Facilitates Neoplastic Growth through a Granulocytic Myeloid-Derived Suppressor Cell-Dependent Mechanism (original) (raw)
Figure 3
Gene expression analysis of CD11b+Gr-1high myeloid cells from G-CSF-treated mice reveal remarkable similarities with those of 4T1 tumor-bearing mice.
(A) Genome-wide mRNA microarray studies were conducted on purified splenic CD11b+Gr-1high cells isolated from three groups of BALB/c mice: non-tumor-bearing control (WT), G-CSF treated (GCSF) or 4T1 tumor-bearing (TB) mice. The number of genes differentially expressed (≥2-fold change; P<0.01) for each of the indicated comparisons is shown in bar plot. There were 932, 734 and 22 genes showing significant expression change in 4T1-TB vs. WT, GCSF vs. WT, and 4T1-TB vs. G-CSF respectively. The fraction of genes up- or down-regulated are shown in gray or black, respectively. (B) Hierarchical clustering of differentially expressed (≥2-fold change; P<0.01) genes from comparisons for 4T1-TB vs. WT, G-CSF vs. WT, and 4T1-TB vs. G-CSF respectively. The color scale of heat map represents the relative expression level of a gene (i.e., red, increased; green, decreased) across the samples.