The C Proteins of Human Parainfluenza Virus Type 1 Block IFN Signaling by Binding and Retaining Stat1 in Perinuclear Aggregates at the Late Endosome (original) (raw)
Figure 1
Signaling in WT or F170S HPIV1-infected Vero cells following treatment with IFN-α, -β, or -γ, assayed by VSV-GFP plaque formation.
Vero cells were mock-infected or infected with WT or F170S HPIV1 at an MOI of 5 TCID50/cell. After 48 h, cells were mock-treated or treated with 100 or 1000 IU/ml of the indicated IFN for 24 h. Subsequently, cells were infected with GFP-expressing VSV, and VSV plaques were enumerated 48 h later. Inhibition of VSV plaque formation is an indication of IFN signaling to create an antiviral state. The relative plaque numbers are shown, as percent of the number of plaques that formed in non-IFN-treated wells. WT and F170S HPIV1-infected cells stimulated with 100 or 1000 IU of any of the three IFNs differed significantly in their ability to restrict VSV plaque formation (P values for two-tailed T-tests are indicated).