A Real-Time PCR Antibiogram for Drug-Resistant Sepsis (original) (raw)
Figure 3
The real-time PCR antibiogram for pan-bacterial detection and identification.
a) Pan-bacterial amplification illustrates universal binding of the primers and b) melt curve analysis provides evidence that consistent, species-specific amplicons were generated. c) Amplicons run on gel electrophoresis exhibit similar lengths of 200-300 base pairs and d) sequences submitted to the BLAST are readily identifiable.