Angiogenin Enhances Cell Migration by Regulating Stress Fiber Assembly and Focal Adhesion Dynamics (original) (raw)
Figure 5
Down-regulation of ANG increased the formation of focal adhesions.
(A) HeLa cells were treated with control siRNA, ANG siRNA 1 or ANG siRNA 2 for 48 h, then fixed and stained with rhodamine-phalloidin (red) and anti-paxillin antibody (green). Nuclei stained with DAPI (blue). (B) Numbers of paxillin-staining focal adhesions was quantified for control and ANG knockdown cells using ImageJ software. Seven cells were analyzed per condition in each experiment. Three independent experiments were performed. **P<0.01. (C) Average size of paxillin-containing focal adhesions in control and ANG-deficient cells. **P<0.01. (D) HUVECs were treated with control siRNA or ANG siRNAs, fixed, and stained with rhodamine-phalloidin (red) and anti-paxillin antibody (green). Nuclei were stained with DAPI (blue).