The AAA-ATPase VPS4 Regulates Extracellular Secretion and Lysosomal Targeting of α-Synuclein (original) (raw)
Figure 5
VPS4 was found to be a component of Lewy body.
Paraffin embedded sections including the substantia nigra and the temporal lobes from four patients with PD with a mean age of 77.5 years and the controls with a mean age of 77.3 years were subjected to immunohistochemical analysis using anti-human VPS4 Ab. Diaminobenzidine were used to visualize the staining and nuclei were counterstained with hematoxylin. In all brain tissues from patients with PD, the core structures of Lewy bodies showed VPS4 immunoreactivity (Fig. 5). Only weak background staining was observed in control brain sections (data not shown). The percentage of VPS4-immunoreactive Lewy bodies in the substantia nigra (A and B) and the temporal lobes (C and D) of PD brains were 90% and 10%, respectively. Scale bar: 20 µm.