Foxa1 Reduces Lipid Accumulation in Human Hepatocytes and Is Down-Regulated in Nonalcoholic Fatty Liver (original) (raw)
Figure 4
Foxa1 reduces lipid accumulation and ApoB100 secretion in human liver cells.
(A) Cultured human hepatocytes and HepG2 cells were transduced with Ad-Foxa1 or Ad-Control for 24 h and then exposed to FA (oleate: palmitate, 2∶1). Twenty-four hours later, accumulated lipids were quantified by Nile Red fluorescence. Data represent the mean±SD of 4–5 independent experiments. * p<0.05; ** p<0.01; *** p<0.001. (B) Left panels: Representative microphotographs of living HepG2 cells and human cultured hepatocytes showing cytoplasmic neutral lipids stained with BODIPY 493/503 (green), nuclei stained with Hoechst 33342 (blue) and death cell nuclei stained with propidium iodide (pink). Right panels: Quantification of the BODIPY 493/503 fluorescent signal with the Olympus ScanR Screening Station in HepG2 cells (mean±SD, n = 4) and in a representative human hepatocyte culture. (C) Secreted (culture medium) and intracellular ApoB100 protein was analyzed by immunoblotting at 48h after infection with adenoviral vectors. (M, fresh culture medium; Cont, non-infected controls; Ad-C, Ad-Control; Ad-F, Ad-Foxa1).