Foxa1 Reduces Lipid Accumulation in Human Hepatocytes and Is Down-Regulated in Nonalcoholic Fatty Liver (original) (raw)
Figure 5
Foxa1 decreases FA uptake.
(A) Cultured cells were infected with Ad-Foxa1 or Ad-Control for 48h, and the mRNA level of the plasma membrane fatty acid transporter protein 2 (FATP2) was measured by real-time Q-RT-PCR. (B) Human hepatocytes and HepG2 cells were infected with Ad-Foxa1 for 24h and were then exposed to 0.2 mM FA (oleate: palmitate, 2∶1) for an additional period lasting 16 h. The amount of non-consumed (residual) free FA, expressed as the percentage of the initial amount in the culture medium, were determined with a specific kit. (C) The uptake of a fluorescent FA analog (C1-BODIPY 500/510-C12) was measured in HepG2 cells and human cultured hepatocytes with an Olympus ScanR Screening Station. Data represent the mean±SD of 4–5 independent experiments. * p<0.05; ** p<0.01; *** p<0.001 in relation to Ad-Control infected cells.