Exendin-4 Improves Steatohepatitis by Increasing Sirt1 Expression in High-Fat Diet-Induced Obese C57BL/6J Mice (original) (raw)
Figure 4
Histopathology of mice fed control (low fat), HF, HF+exendin-4 diet for 10 wks.
Sections were stained with H&E. Arrows indicates lipid droplets. (A–C) Liver sections of mice fed control, HF and HF+Ex-4, respectively. (magnification, ×200). Scale bars = 500 µm. (D) NAFLD activity scores were evaluated semi-quantitatively: steatosis (0–3), lobular inflammation (0–2), and hepatocellular ballooning (0–2). N.D., not detected (E) Hepatic triglycerides were extracted from frozen tissue and measured by enzymatic assays. Values of TG were normalized to protein concentration. * p<0.05, ** p<0.01 compared with a control and # p<0.05, ## p<0.01 compared with a HF.