GUCY2C Opposes Systemic Genotoxic Tumorigenesis by Regulating AKT-Dependent Intestinal Barrier Integrity (original) (raw)
Figure 1
GUCY2C regulates intestinal barrier integrity.
(A) Comparison of mRNA expression of genes associated with the canonical tight junction pathway (KEGG 04530) of Gucy2c+/+ and Gucy2c−/− mice (n = 4, p = 0.029). (B) Immunoblot analysis of tight junction proteins from the distal colon of 12 week old Gucy2c+/+ and Gucy2c−/− mice. Data represent means of n≥7±SEM. (C) Immunofluorescence staining of claudin 4 from the distal colon of 12 week old Gucy2c+/+ and Gucy2c−/− mice. (Nucleus-DAPI, β-catenin-red, Claudin 4-green; 1,000×). (D) Transmission electron microscopy of tight junction complexes from jejuna of 12 week old Gucy2c+/+ and Gucy2c−/− mice (25,000×). (E) FITC-dextran was administered by oral gavage to age-matched Gucy2c+/+ and Gucy2c−/− mice after an overnight fast. Serum fluorescence was analyzed 90 min after gavage. One representative experiment of three (n = 3). *, p<0.05, **, p<0.01.