3T3 Cell Lines Stably Expressing Pax6 or Pax6(5a) – A New Tool Used for Identification of Common and Isoform Specific Target Genes (original) (raw)
Figure 5
Real time quantitative PCR shows that Dkk3, Ncam1, Ngef and Sphk1 are strongly regulated by Pax6(5a).
Primers were designed for some of the genes showing differential expression only in the FlpIn-3T3 Pax6(5a) cell line (Dkk3, Hist1h1c, Nbl1, Ncam1, Ngef, Pdgfb, Sphk1). Real time quantitative PCR was performed as described in Fig. 4. The fold change values obtained for the same genes in the Illumina gene expression microarray are given in the table to the left of the graph for comparison.