3T3 Cell Lines Stably Expressing Pax6 or Pax6(5a) – A New Tool Used for Identification of Common and Isoform Specific Target Genes (original) (raw)
Figure 6
Chromatin immunoprecipitation verifies binding of Pax6 and Pax6(5a) to the promoter region of several target genes.
Chromatin immunoprecipitation with an antibody against Pax6 was done for both the 3T3-Pax6 and the 3T3-Pax6(5a) cell line. IgG was used as a control. PCR primers were designed so that they should amplify target sequences with putative Pax6 and/or Pax6(5a) binding sites in the promoter regions of Crtap, Ctgf, Edn1, Pdgf, Dkk3 and Ngef. The number in parenthesis specifies the identity of the primerset used for the observed amplification. Genomic DNA sampled before adding the antibody (input) was used as a positive control for the PCR reaction, while water was used as a negative control. The promoter region of Gapdh was included as a region not expected to bind Pax6 or Pax6(5a).