Prevalence and Genetic Characterization of Pertactin-Deficient Bordetella pertussis in Japan (original) (raw)
Figure 1
Prn expression in B. pertussis clinical isolates.
The isolates harboring prn2 allele (BP157, BP159, BP162, BP228, and BP235) and prn1 allele (BP155, BP156, BP232, BP233, and BP243) were cultured on CSM plates. Total protein (10 µg) extracted from the bacterial cells was separated by SDS-PAGE followed by CBB R-250 staining (left panel). Immunoblots (1 µg protein/lane) were incubated with anti-Prn1, anti-PT or anti-FHA antiserum (right panel). Ten ng of purified Prn1, PT, or FHA and total protein (1 µg) from B. pertussis Tohama were run on the gel as positive controls.