Dextran Sodium Sulfate (DSS) Induces Colitis in Mice by Forming Nano-Lipocomplexes with Medium-Chain-Length Fatty Acids in the Colon (original) (raw)
Figure 7
DSS and fatty acids form nano-lipocomplexes that disrupt Caco2-BBE monolayer resistance.
(A) Confluent cultures were obtained after 48 h of growth and epithelial resistance was assessed by continuous resistance measurement at pH 7.4 (cellular pH) or pH 6.2 (colonic pH). The epithelial resistances of cultures treated with dodecanoate alone (10 mM) or with 3% (w/v) DSS (1 or 10 mM dodecanoate), at pH 6.2 or pH 7.4, were assessed by ECIS. (B) Light scattering measurement of the diameters (nm) of particles formed by DSS (3% w/v) alone; DSS (3% w/v) with Ca++ (1 mM); and DSS (3% w/v) with Ca++ (1 mM) and butyrate (1 or 10 mM) or dodecanoate (1 or 10 mM).