A Pivotal Role of Vitamin B9 in the Maintenance of Regulatory T Cells In Vitro and In Vivo (original) (raw)
Figure 1
Requirement of vitamin B9 for the maintenance of Treg cells.
(A) Purified naïve CD4+ T cells were stimulated with anti-CD3 and anti-CD28 antibodies plus TGF-β in the presence of normal [Vit B9(+)] or reduced [Vit B9(−)] amounts of vitamin B9. After 4 days, total cell numbers were calculated, and the differentiation into Foxp3+ Treg cells was examined by flow cytometry. Data are means ± SEM (n = 4). (B) CD25+CD4+ T cells were cultured with anti-CD3 antibodies in Cont or B9(−) medium. The frequencies of Foxp3+ and Foxp3−CD4+ T cells (B) were determined by flow cytometry. Cell numbers were calculated using the total cell number and flow cytometric data. Data are means ± SEM (n = 6). (C) Experiments similar to that shown in (B) were performed with different concentrations of vitamin B9. The relative cell number of Foxp3+ Treg cells is expressed as a ratio to the cell number in control medium. The values and means are indicated with dots and lines, respectively. Similar results were obtained from 2 independent experiments.