Micro-RNA Profiling in Human Serum Reveals Compartment-Specific Roles of miR-571 and miR-652 in Liver Cirrhosis (original) (raw)
Figure 5
miR-571 and miR-652 integrate pro-fibrotic and inflammatory signals in human HSCs and monocytes.
(A) Expression of miR-571 and miR-652 in primary human HSCs in response to stimulation with TGF-β for 48 h was measured by qPCR. Results are depicted as dot plot with each dot representing one individual patient. The line represents the mean of relative expression. (B) We stimulated U937 cells with PMA into a monocytic differentiation. miR-571 and miR-652 expression levels in response to stimulation with LPS for 48 h were measured by qPCR. Results are depicted as dot plot with each dot representing one individual patient. The line represents the mean of relative expression. (C) Expression of CREBBP was analyzed by qPCR in liver samples of patients with liver cirrhosis (n = 13) and non-liver diseased donors (n = 10) as control. Results are depicted as mean, error bars denote SEM. (D) HuH-7 cells and LX-2 cells were transfected with indicated amounts of miR-571 mimic. Expression of CREBBP was determined after two days of transfection by qPCR. Results are depicted as mean, error bars denote SEM. (E) Activation of stellate cells was determined by qPCR-based analysis of SMA- and Col1A1-expression in LX-2 cells. **P<0.01, ***P<0.001.