Unique Proteomic Signatures Distinguish Macrophages and Dendritic Cells (original) (raw)
Figure 2
The plasma membrane proteome of bone marrow-derived dendritic cells (DCs).
Panel A: Bone marrow-derived dendritic cells (BmDCs) were obtained by culturing bone marrow cells with GM-CSF. Panel B: Flow cytometric analysis of CD11c and F4/80 expression in BmDCs and BmMs. Results are presented as a contour plot with 10% probability increments. Panel C: Cell-surface exp ression of MHC-II by BmDCs and BmMs as assessed by flow cytometry. Panel D: The plasma membrane proteome of DCs. Upper Panel: Proteins expressed at similar levels by DCs and either M1 cells, M2 cells, or BmMs. Lower Panel: Proteins differentially expressed by DCs relative to M1 cells, M2 cells, and BmMs (_G-_test>1.5 or <−1.5 and _t-_test: p<0.05). Red, upregulated; green, downregulated. Panel E: Examples of proteins expressed at similar levels by DCs and either M1 cells, or M2 cells. Results (N = 6 per group) are means and SDs. Panel F: Examples of plasma membrane proteins differentially expressed by DCs. Flow cytometry experiments are representative of 3 independent analyses.