Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection (original) (raw)
Figure 3
Expression and purification of recombinant pET-26b-_Cs_Pmy identified by 8% SDS-PAGE.
(A) Expression of pET-26b-_Cs_Pmy. Protein molecular weight marker (M), lysate of E. coli with pET-26b(+) before induction with IPTG (lane 1) and after induction (lane 2), lysate of E. coli with pET-26b-_Cs_Pmy before induction with IPTG (lane 3) and after induction (lane 4), supernatant of induced E. coli with pET-26b-_Cs_Pmy (lane 5) and sediment (lane 6). (B) Denaturation of inclusion bodies containing pET-26b-_Cs_Pmy. Supernatant collected from inclusion bodies dissolved in 2 M urea (lane 1) and 6 M urea (lane 2). (C) Purification of pET-26b-_Cs_Pmy. Protein eluted with 40 mM imidazole (lane 1–3), 80 mM imidazole (lane 4–8), 100 mM imidazole (lane 9–12), 200 mM imidazole (lane 13–14). Proteins were visualized by Coomassie Blue staining, the protein bands were around 100 kDa.