Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection (original) (raw)
Figure 6
Immunohistochemical localization of _Cs_Pmy at adult worm and metacercaria.
Adult worms and metacercariae of C. sinensis were fixed with 4% paraformaldehyde, embedded with paraffin and sliced into 3–5 µm in thick. The sections were blocked with normal goat serum overnight at 4°C, and then incubated with primary antibody (1∶200 dilutions) at room temperature for 2 h. After washing procedures, the sections were incubated with goat anti-rat IgG Alexa Fluor 594 (1∶400 dilutions) at room temperature for 1 h in dark. The images were captured under fluorescence microscope (ZEISS, Goettingen, Germany). Panel A–H, adult worm of C. sinensis. Pane I–L, metacercariae of C. sinensis. Pane A, B, E, F, I and J were sections treated with anti-pET26b-_Cs_Pmy serum. C, D, G, H, K and L were sections treated with naïve serum and imaged under the same conditions. Specific immunofluorescence was indicated in red (pane A, E and I), while no immunofluorescence was detected in pane C, G and K. Corresponding white light of parasite was panel B, D, F, H, J and L. T, tegument. OS, oral sucker. V, vitellarium. CW, cyst wall. EB, excretory bladder. Magnification for adult worm and metacercaria were ×100 and ×400, respectively.