SCD1 Inhibition Causes Cancer Cell Death by Depleting Mono-Unsaturated Fatty Acids (original) (raw)
Figure 4
SCD1 inhibition is not universally toxic, and slows growth of HCT116 xenograft tumors.
A HCT116 or SKOV3 cells were treated and analyzed for cell viability or cellular SCD1 inhibition (LC/MS/MS) as described above. B HCT116 or SKOV3 cells were treated and analyzed for cell viability. Table expresses the ratio of SKOV3 EC50 versus HCT116 EC50. C, D Nude mice harboring passage five 200 mm3 HCT116 tumors (passaged as trocar fragments) (n = 10 per group) were dosed by oral gavage twice daily with 160 mg/kg #28c for 20 days or with intravenous CPT11 on three consecutive days starting when tumors reached 200 mm3. Tumor growth (C) and body weight (D) were monitored and plotted as mean +/− standard deviation.