Angiotensin Converting Enzyme (ACE) and ACE2 Bind Integrins and ACE2 Regulates Integrin Signalling (original) (raw)
Figure 4
ACE2 affects cellular adhesion in vitro.
Huh7 cells were loaded with BCECF and plated on to monolayers of HEK, HEK-ACE2 or HEK-tACE cells, unadhered cells were washed off. A) Cell adhesion was examined using fluorescence microscopy, Panel i, HEK and HEK-ACE2 substrates were of equal confluence. Panel ii, labelled cells were visualised at 450–480 nm excitation wavelength. B) Fluorescent intensity increases linearly with the number of cells plated. Serial dilutions were made of the labelled Huh7 cells and fluorescence was read. C) Cellular expression of ACE2 increases cell adhesion independent of RGD motif. Cells were treated as before or the HEK-ACE2 cells were pre-incubated with RGD peptide before Huh7 cell addition. The number of cells which had adhered was quantified by fluorescent emission. D) Cellular expression of tACE does not affect cell adhesion. Cells were treated as before, the number of cells which had adhered was quantified by fluorescent emission.