Mechanism of Chemical Activation of Nrf2 (original) (raw)

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Figure 1

CPDT and SF stimulate Nrf2 transactivation activity by stabilizing its protein.

(A) Chemical structure of CPDT and SF. RT-4 cells and NBT-II cells were treated with CPDT (50 µM), SF (8 µM) or vehicle (0.1% DMSO) for 6 or 24 h. (B) Whole cell lysates were then prepared for IB or (C) total RNA was isolated for RT-PCR analysis. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a control.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0035122.g001