Characterization of a Novel ArsR-Like Regulator Encoded by Rv2034 in Mycobacterium tuberculosis (original) (raw)
Figure 6
EMSA assays for the ability of Rv2034 to bind variant DNA substrates.
(A) A diagram showing the DNA substrates used in this assay. The DNA substrates are 40 bp or 44 bp long, and the binding sites in them are shown in bold. The inverted repeats (IR) and the “spacer” sequence between inverted repeats are indicated on the top. Sequences are aligned and the mutated region(s) are indicated in red. (B) EMSA assays for the DNA-binding activity of Rv2034 on the DNA substrates listed in (A). Each DNA substrate was co-incubated with 0, 0.1, 0.2, 0.4, 0.8, 1 and 1.2 µM of Rv2034 proteins (final concentration for lanes 1–7, respectively).