CRISPR Typing and Subtyping for Improved Laboratory Surveillance of Salmonella Infections (original) (raw)
Figure 4
Overview of the bead-based CRISPOL assay for S. enterica serotype Typhimurium developed here.
The estimated time for each step is based on the testing of 65 isolates in a 96-well plate. The amplification step spans from the preparation of thermolysates to the gel electrophoresis of PCR products.