Bone Marrow Mesenchymal Stromal Cells Stimulate Skeletal Myoblast Proliferation through the Paracrine Release of VEGF (original) (raw)
Figure 2
Assessment of myoblast cell proliferation by cyclin A expression.
C2C12 cells in single and co-culture with Dil(red)- or GFP(green)-labeled MSCs for 24 h were incubated with specific antibodies against cyclin A (green, A,B; red, D-G) and observed by confocal microscopy. Notably, the cells with the higher immunofluorescence intensity are those located in close contact with MSCs. C) Quantitative analysis of the number of cells positive for cyclin A. Data represent the results of at least three independent experiments with similar results. *p<0.05.