Expression of Transient Receptor Potential Ankyrin 1 (TRPA1) and Its Role in Insulin Release from Rat Pancreatic Beta Cells (original) (raw)
Figure 4
TRPA1-mediated insulin release in pancreatic beta cell line and primary isolated pancreatic islets.
a,b. Dose-dependent increase in insulin release induced by AITC (0.1–1000 µM, n=7) and MG (0.1–1000 µM, n=5) in RIN cells (* p<0.05). c,d. AITC and MG induce a significant increase (AITC, n=11,* p<0.001; MG, n=10 * p=0.004) in insulin release from primary isolated pancreatic beta cell islets that could be blocked by the specific TRPA1 antagonist AP-18 (AITC+AP-18, n=6, ** p<0.001; MG+AP-18, n=6, ** p=0.008). e. 4-HNE (100 µM)-induced insulin release is inhibited by HC030031 (100 µM) (4-HNE, n=6, * p<0.001; 4-HNE+HC030031, n=3, ** p<0.001). f. PGJ2 (20 µM)-induced insulin release is inhibited by HC030031 (100 µM) (PGJ2, n=6, * p<0.001; PGJ2+HC030031, n=3, ** p<0.001).