Beyond Repair Foci: DNA Double-Strand Break Repair in Euchromatic and Heterochromatic Compartments Analyzed by Transmission Electron Microscopy (original) (raw)
Figure 1
Gold-labeled pKu70 and 53BP1 in cortical neurons of brain analyzed 40 min after irradiation with 6Gy.
TEM micrographs of double-labeling of pKu70 (10-nm beads) and p53BP1 (6-nm beads) at different magnifications (boxed regions are shown at higher magnifications in the following images). Complex pKu70 clusters (consisting of 4 gold beads) co-localizing with 53BP1 were observed in heterochromatic regions, but only isolated pKu70 clusters without any p53BP1 binding were observed in euchromatic regions (pKu70 beads are marked by red arrows).