Beyond Repair Foci: DNA Double-Strand Break Repair in Euchromatic and Heterochromatic Compartments Analyzed by Transmission Electron Microscopy (original) (raw)

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Figure 2

Gold-labeled pKu70 and 53BP1 in mouse tissues and human fibroblasts analyzed 40 min after irradiation with 6Gy.

TEM micrographs of double-labeling of pKu70 (10-nm beads) and p53BP1 (6-nm beads) at different magnifications. In enterocytes of small intestine, keratinocytes of skin as well as in human fibroblast, radiation-induced pKu70 clusters (consisting of 2 gold beads, red arrows) co-localize with 53BP1 only in heterochromatic regions.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0038165.g002