Vibrio cholerae vexH Encodes a Multiple Drug Efflux Pump That Contributes to the Production of Cholera Toxin and the Toxin Co-Regulated Pilus (original) (raw)
Figure 2
Infant mouse colonization assays with the RND efflux mutants.
Competition assays were performed using the infant mouse colonization assay as described in the Materials and Methods. Infant mice were challenged with a ∼105 cfu inoculum containing a mixture of wild-type and the indicated mutant at a ratio of 1∶1 (A) or 1∶100 (B). The competitive index was calculated as the ratio of mutant to wild-type recovered from the small intestine, corrected for the ratio of mutant to wild-type that was present in the inoculum. Each symbol represents one mouse. *The ΔRND mutant was not recovered from mice necessitating the calculation of a theoretical CI as described in the Materials and Methods. Mean and standard deviation are indicated by horizontal bars. Significance was determined using the Mann-Whitney U t-test. (1) p<0.01.