Impaired LDL Receptor-Related Protein 1 Translocation Correlates with Improved Dyslipidemia and Atherosclerosis in apoE-Deficient Mice (original) (raw)
Figure 5
Reduced atherosclerosis development in ApoE−/−LRP1n2/n2 mice.
A–B, Spontaneous atherosclerosis development in 26- (A) and 52-week (A–B) old mice. C–E, Different cholesterol levels in lipoprotein fractions (VLDL, LDL and HDL) separated via sequential ultracentrifugation in mice at 52-weeks of age (C), total triglyceride levels (D) and correlation plot between atherogenesis and total cholesterol for individual mice (E). Statistical analysis via determination of the Pearson’s correlation coefficient (Rp) revealed a significant positive correlation between atherosclerosis load in the aorta and circulating cholesterol levels. F, Total plasma cholesterol levels in mice at 12-, 26- or 52-weeks of age. G, Immunoblot analyses of hepatic LDLR and β-actin expression levels in 8- and 52-week old mice. ApoE−/− (□ or ○) and apoE−/−LRP1n2/n2 (▪ or •) mice, n = 7–12 on a chow diet, data are mean±SEM. *P<0.005, **P<0.0005.