Mesenchymal Cell Interaction with Ovarian Cancer Cells Triggers Pro-Metastatic Properties (original) (raw)
Figure 2
MCs increase OCC metastasis biological function: adherence, migration and invasion.
A. OCC-eGFP (NIH:OVCAR3, left chart and SKOV3, right chart) were seeded on a Matrigel (BD Biosciences)-coated well for 10 min, 15 min, 30 min and 1 hour. Increased adherence to the ECM is observed when OCC were preemptively cultivated with MC (light grey bars) compared to the control (dark grey bars). B. Scheme representing the invasion and migration assay. C. Sorted OCC were seeded on (un) coated transwells and GFP signal of each well under the coated membrane was acquired after 24 h. Increased migration and invasion through the ECM is observed when OCC were preemptively cultivated with MC (light grey bars) compared to the control (dark grey bars). SEM are represented, n = 3, | p<0.05 t-Student Test.