Coaggregation of RNA-Binding Proteins in a Model of TDP-43 Proteinopathy with Selective RGG Motif Methylation and a Role for RRM1 Ubiquitination (original) (raw)

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Figure 10

A methylated RGG motif peptide increasing with TDP-43 overexpression identified by unlabeled/SILAC peptide comparison.

(A) MS/MS of an hnRNP A/B dimethylated R332 peptide, both unlabeled (upper panel) and heavy (lower panel). (B) MS spectrum for the peptide in (A) at the LC elution peak from which ion intensities for light and heavy peptides were extracted to calculate Table 4 unnormalized quantified relative levels of the modification in mock, TDP-43, and TDP-S6 paired experiments. Replicate 1 (urea/urea mixture) spectra are shown. (C) A representative unmodified peptide for hnRNP A/B (IFVGGLNPEATEEK), the quantified relative levels of which contributed to the normalization factors in Table 2.

Figure 10

doi: https://doi.org/10.1371/journal.pone.0038658.g010