Activation of Pregnane X Receptor by Pregnenolone 16 α-carbonitrile Prevents High-Fat Diet-Induced Obesity in AKR/J Mice (original) (raw)
Figure 3
Effect of PCN treatment on glucose tolerance, insulin sensitivity, serum concentration of insulin, and mRNA level of G6Pase and Pepck.
Animals at the end of the 7-week treatments were fasted overnight for glucose tolerance tests, or fasted for 4 h for insulin sensitivity tests. A, time-dependent blood concentration of glucose upon IP injection of glucose (2 g/kg); B, area under the curve from glucose tolerance test in A; C, time dependent glucose concentration upon IP injection of insulin; D, insulin levels at the end of the 7-week feeding with high-fat diet and regular chow food with or without PCN treatment; E, relative mRNA level of G6Pase and Pepck in mouse liver at the end of animal feeding and PCN treatment; and F, HOMA-IR values calculated based on formula: Glucose (mg/dl) × Insulin ( µU/ml)/405. Each data point represents the average ± SD of 4 animals in each group. #P<0.05, ##P<0.01 compared to chow-DMSO group; *P<0.05, **P<0.01 compared to high-fat diet-DMSO group.