Epstein-Barr Virus Immortalization of Human B-Cells Leads to Stabilization of Hypoxia-Induced Factor 1 Alpha, Congruent with the Warburg Effect (original) (raw)
Figure 4
HIF1A is localized to the nucleus and cytoplasm in LCLs, in contrast to mitogen-activated B-cells.
A – Western blotting of cellular subfractions. The membrane was probed with mouse anti-HIF1A and anti-actin antibodies (left panel). Nuclear HIF1A was detected in the lysates of LCL; however, no nuclear signal for HIF1A was detected in mitogen-activated cells. The HIF1A/actin ratio for all the probes was calculated (right panel). B – Immunostaining of LCLs and mitogen-activated cells with mouse anti-HIF1A antibody (green signal). Nuclear DNA is stained in blue. Notice the presence of nuclear HIF1A signal in LCL (panels a and b) and its absence in the mitogen-activated cells (c and d).