Classical Macrophage Activation Up-Regulates Several Matrix Metalloproteinases through Mitogen Activated Protein Kinases and Nuclear Factor-κB (original) (raw)
Figure 3
Activation of MAP kinases and effects of inhibitors.
M-CSF differentiated macrophages derived from unselected human CD16+/− monocytes (M0) were pre-treated for 45 min with 10 µM PD98059, SB20380, SP600125 or vehicle (0.1% v/v DMSO) and then some were classically activated with LPS and IFNγ (M1). Phospho and total proteins were measured by western blotting. Levels were normalised to total proteins levels. (A) ERK1/2 with and without PD98059, (B) p38 MAPK with and without SB20380, (C) JNK with and without SP600125, (D) Steady-state mRNA levels of MMPs and other genes were measured after 18 hours of stimulation with LPS and IFNγ. Values are log weighted means and 95% confidence intervals * p<0.05 compared to without inhibitor (n = 7).