Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue (original) (raw)
Figure 2
Scatterplots of RefSeq transcript abundance.
Log10 read counts are plotted. Transcripts with zero reads are excluded from analysis. An identity line is shown in each plot for reference. Sub-populations are indicated as follows: Black cross – sno-, sc-, sn- and mir-RNAs from RefSeq; Black and red circles – Genes homologous to SDRNA and SDRNA2 probes, respectively (Table S4); Light blue circles – All other RefSeq transcripts. FF – Fresh-frozen; FFPE – Formalin-fixed, paraffin-embedded. (a) FFPE RNA Pool: SDRNA1 vs SDRNA2. (b) FF breast RNA : polyA+ vs SDRNA1. (c) FF breast RNA : polyA+ vs SDRNA2.