Low Bone Turnover and Low BMD in Down Syndrome: Effect of Intermittent PTH Treatment (original) (raw)
Figure 2
Bone Formation and bone turnover is decreased in Ts65Dn Mice.
(A). Representative double-fluorochrome-labeled regions of trabecular bone in the proximal tibia of 3 month old WT (top) and Ts65Dn (bottom). Arrowheads indicate the distance between the two labels used to calculate the bone formation rate normalized to bone surface (BFR/BS) (um3/um2/day). (B). Tibial sections (N = 6 per group) were used to quantify BFR/BS, percent osteoblast surface/bone surface (Ob.S./BS), percent osteoclast surface/bone surface (Oc.S./BS) and number of osteoclasts/bone surface (N.Oc./BS.) (C). Serum obtained from WT and Ts65Dn mice at 3- and 24-months of age was analyzed for bone resorption, tartrate resistant acid phosphatase 5b (TRAP 5b) and bone formation, procollagen-1 N-terminal peptide (P1NP) markers by ELISA. *, p<0.05 vs. respective WT vehicle control.