A Large Insertion in bHLH Transcription Factor BrTT8 Resulting in Yellow Seed Coat in Brassica rapa (original) (raw)
Figure 3
Differences in DNA and mRNA expression.
(A) The insertion location is shown in gray rectangles in the ORF of the BrTT8. The black rectangles represent the exons, and TL1, TL2, TR1 and YCR1 are the primers that were developed from the corresponding exon sequences and insert sequence. The arrows are used to indicate the directions of the primers. The insertion sequences and flanking BrTT8 intron 2 are shown in red and black, respectively. The conserved sequences at the termini of the element are underlined. Palindromic sequences that are capable of forming a hairpin are shown in blue. (B) The amplification products of the genomic DNA of the yellow-seeded (1) and black-seeded line (2) using the primers TL1 and TR1. (C) The primers TL2, TR1 and YCR1 amplified the genomic DNA from the three genotypes: (1) the homozygous yellow-seeded, (2) heterozygous black-seeded, (3) homozygous black-seeded plants. (D) mRNA levels in the immature seeds of the yellow-seeded line (three on the left) and black-seeded line (three on the right). The numbers 10, 20, and 30 signify the number of days after pollination. (E) 18S control.