The PICALM Protein Plays a Key Role in Iron Homeostasis and Cell Proliferation (original) (raw)
Figure 6
PICALM-deficient MEFs proliferate more rapidly in the presence of iron supplementation and are more sensitive to iron chelation.
(A,B) Representative proliferation curves of non-immortalized _Picalm_NULL and WT MEFs without (A) and with (B) 50 µM ferric ammonium citrate (FAC). FAC restores proliferation in _Picalm_NULL cells to levels in WT cells. (C,D) Mean cell numbers (+/− standard error) of non-immortalized _Picalm_NULL and WT MEFs grown for 5 days without (C) and with (D) 50 µM FAC. Nexp = 3. *p<0.04 compared with WT cells. (E,F) Representative proliferation curves of immortalized _Picalm_NULL MEFs transduced with empty vector (control) or PICALM grown for 4 days in the absence (E) or presence (F) of 2.5 µM deferoxamine (DFO). (G) Mean cell number of _Picalm_NULL MEFs untransduced (Null 3T) or transduced with empty vector (control) or PICALM after 4 days of culture in the presence of 2.5 µM DFO treatment relative to number of untreated cells. Nexp = 4. *p<0.003 compared with PICALM rescued cells.