T Cell Responses Induced by Adenoviral Vectored Vaccines Can Be Adjuvanted by Fusion of Antigen to the Oligomerization Domain of C4b-Binding Protein (original) (raw)
Figure 2
Adjuvant activity of IMX313 following immunization with AdHu5-PfAMA1 or AdHu5-PfM128.
BALB/c mice were immunized i.m. with 1×109 ifu AdHu5-PfAMA1±IMX313 (AdAMA1-IMX313 and AdAMA1-Nil). Twenty-one days post-immunization frequency of IFN-γ, TNFα and IL-2 positive CD8+ and CD4+ T cells was assessed in the blood by ICS following stimulation with PfAMA1 peptides (A). Antigen-specific total IgG was assessed by ELISA (B). Points represent individual mice and bars indicate median responses (n = 5–6 mice per group). **P<0.01, *P<0.05 compared to AdAMA1-IMX313 by Mann Whitney test. Data show the results of a single experiment. A similar result was observed by spleen IFN-γ ELISPOT (Figure S3). In a separate experiment, mice were immunized i.m. with 1×107 ifu AdHu5-PfM128±IMX313 (AdPfM128-IMX313 and AdPfM128-Nil). Fourteen days later mice were culled and PfMSP133-specific IFN-γ positive splenocytes were measured by ELISPOT (C). Data shown are spot forming units (SFU) per 106 splenocytes. PfMSP119-specific total IgG was assessed by ELISA (D). Bars indicate median responses (n = 5 mice per group). Box and whisker plots indicate median, IQR and range. The dashed line indicates the limit of detection. + P<0.05 compared to AdPfM128-IMX313 by Mann Whitney test.