T Cell Responses Induced by Adenoviral Vectored Vaccines Can Be Adjuvanted by Fusion of Antigen to the Oligomerization Domain of C4b-Binding Protein (original) (raw)
Figure 6
Adjuvanticity of IMX108 in FcRγ−/− mice.
Wildtype or FcRγ−/− mice were immunized i.m. with 1.5×109 ifu Ad42-IMX108 or Ad42-Nil. Two weeks later PyMSP133-specific IFN-γ, TNFα and IL-2 positive CD8+ T cells in the spleen were measured by ICS (A). Box and whisker plots show median, IQR and range for n = 7–13 mice/group. PyMSP119-specific total IgG was measured by ELISA (B). Points indicate individual mice and bars indicate median titer. n = 6 mice/group. *P<0.05, **P<0.01, by Mann Whitney test. Combined data are shown from two independent experiments.