Human P301L-Mutant Tau Expression in Mouse Entorhinal-Hippocampal Network Causes Tau Aggregation and Presynaptic Pathology but No Cognitive Deficits (original) (raw)
Figure 7
Tau aggregates in the DG of 16-month-old EC-hTau mice.
(A–E) Gallyas silver staining revealed no abnormalities in NTG (A) and tet-hTau singly transgenic (B) controls. In contrast, EC-hTau mice had neuropil threads in the outer molecular layer of the DG (C, box 1 enlarged in panel D) and tangle-like inclusions in GC of the DG (box 2 enlarged in panel E). (F) Low magnification (5,000X) view of a GC. (G–H) Higher magnification (30,000X) view of intracellular filamentous aggregates in GC. (I) Immuno-EM analysis of the packed intracellular filaments with the PHF1 antibody. Gold particles decorate straight filaments; gold particles enhanced with silver solution are 15–20 nm. (J) Negative control (no primary antibody) shows the specificity of the immunogold labeling. Scale bars: 30 µm (A, B), 10 µm (D, E), 2 µm (F), 0.5 µm (G, H), 0.1 µm (I) and 0.05 µm (J).